Project

Part:BBa_K2555004:Design

Designed by: Yingqi Ma   Group: iGEM18_BFSUICC-China   (2018-09-20)


PcopA-RiboJ-RBS-sfGFP-PBAD-RBS-CueR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1121
    Illegal SapI site found at 2203
    Illegal SapI.rc site found at 364


Design Notes

L3S3P22,L3S2P11 and L3S2P21


Source

E.coli

References